Analytical Biochemistry
Subject Overview
Analytical Biochemistry covers the core instrumental techniques of the clinical biochemistry laboratory: spectrophotometry and colorimetry (Lambert's and Beer's laws), flame photometry and atomic absorption spectroscopy, the major chromatography types (paper, thin-layer, column, gas, ion-exchange, gel), and electrophoresis. A 4-credit core theory paper.
Unit-wise Syllabus
3 units — click WhatsApp below to get the full notes for each
Unit 1: Spectrophotometry, colorimetry and photometry
Theory of spectrophotometry and colorimetry, Lambert's law and Beer's law, applications; general principles, limitations, instrumentation and applications of flame photometry; atomic absorption spectroscopy principle and applications
Unit 2: Chromatography
Introduction, definition and types of chromatography — paper chromatography, thin-layer chromatography (including HPTLC), column chromatography, gas chromatography, ion-exchange chromatography, gel chromatography — principle, instrumentation and applications of each
Unit 3: Electrophoresis
Introduction, principle, instrumentation, types (paper and gel electrophoresis) and applications