Analytical Biochemistry (Practical) Notes
Subject Overview
The practical companion to Analytical Biochemistry: hands-on demonstration of the spectrophotometer, colorimeter and flame photometer, and of paper, gas, thin-layer and column chromatography and electrophoresis technique. A 2-credit core practical paper.
Subject at a glance
1
Units
7
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Instrumentation demonstrations
- Principle
- working and maintenance of the spectrophotometer
- colorimeter and flame photometer
- principle and procedure of paper chromatography
- gas chromatography
- thin-layer chromatography
- column chromatography and electrophoresis
- 1
Switch on and warm up
- 2
Select the filter
Complementary colour
- 3
Set zero with the blank
- 4
Read the standard
- 5
Read the test
- 6
Calculate concentration
A test / A standard × standard concentration
Study guide
In this practical you study the principle, working and maintenance of the spectrophotometer, colorimeter and flame photometer and demonstrate paper, gas, thin-layer and column chromatography and electrophoresis. Learn the correct sequence for warming up and calibrating each instrument.
Why Analytical Biochemistry (Practical) Matters
Familiarity with laboratory instruments makes you employable in diagnostic and research laboratories from day one.
How to Prepare for Analytical Biochemistry (Practical)
- Learn each instrument's controls, calibration and maintenance routine.
- Record readings systematically.
- Be ready to explain the principle in the viva.
Analytical Biochemistry (Practical): Frequently Asked Questions
What instruments are covered in the analytical biochemistry practical?
Spectrophotometer, colorimeter, flame photometer, and chromatography and electrophoresis setups.