Applied Bacteriology (Practical) Notes
Subject Overview
The practical companion to Applied Bacteriology: performing antimicrobial susceptibility testing end-to-end, collecting and processing water/
Subject at a glance
1
Units
12
Topics
1
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Antimicrobial testing and culture handling
- Antimicrobial susceptibility testing — inoculum preparation and standardization
- reference bacterial strains
- choice of antibiotics
- MIC/MBC determination
- Kirby-Bauer and Stokes methods
- beta-lactamase demonstration
- collection
- transportation and processing of water and air samples
- sterility testing of IV fluids with positive/negative controls
- demonstration of serotyping
- bacteriocin typing and lyophilization
- safe disposal of bacterial cultures
MIC
Lowest concentration with no visible growth
MBC
Lowest concentration that kills 99.9% of bacteria
Bactericidal drug
MBC ≤ 4 × MIC (common rule)
Air sampling (settle plate)
Colonies per plate after a set exposure time
Study guide
In this practical you perform antimicrobial susceptibility testing (inoculum preparation, control strains, antibiotic choice, MIC and MBC, Kirby-Bauer and Stokes, beta-lactamase tests), collect and process water and air samples, test IV fluids for sterility with controls, demonstrate serotyping, bacteriocin typing and lyophilisation and dispose of cultures safely. Standardise the inoculum carefully; it determines the accuracy of the result.
Why Applied Bacteriology (Practical) Matters
Susceptibility testing and sterility testing are routine responsibilities of hospital microbiology laboratories.
How to Prepare for Applied Bacteriology (Practical)
- Standardise the inoculum against the McFarland standard.
- Measure zones of inhibition accurately with a ruler.
- Dispose of cultures following biosafety rules.
Applied Bacteriology (Practical): Frequently Asked Questions
What is done in the applied bacteriology practical?
Antibiotic susceptibility tests, environmental and sterility testing and demonstrations of typing and preservation.