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NotesB.Sc MLSApplied Haematology-I (Practical)
BMLS502-18

Applied Haematology-I (Practical) Notes

Program:B.Sc MLS
Semester:Semester 5
Credits:1
Units:1 Units

Subject Overview

The practical companion to Applied Haematology-I: bone marrow smear and trephine biopsy staining and processing, morphological identification of immature blood cell series, red cell abnormality study, coagulation mixing studies, urine/CSF/seminal fluid examination, and demonstrating blood cell counter and coagulometer function. A 1-credit skill enhancement laboratory paper.

Subject at a glance

1

Units

9

Topics

1

Credits

1

Infographics

Unit-wise Syllabus

1 units — click WhatsApp below to get the full notes for each

1

Unit 1: Bone marrow and body fluid technique

  • Bone marrow smear preparation and staining (Leishman's, May-Grunwald-Giemsa, Perl's)
  • trephine biopsy sectioning and staining (H&E, reticulin, PAS)
  • morphological identification of immature erythroid/myeloid/WBC series
  • red cell abnormality study (size, shape, staining, inclusions)
  • coagulation mixing studies for prolonged PT/APTT/thrombin time
  • physical/chemical/microscopic examination of urine
  • cytological examination of CSF and other body fluids
  • physical/microscopic examination of seminal fluid including sperm count
  • demonstration of blood cell counter and coagulometer function
ProcessMixing study for a prolonged PT or APTT
  1. 1Prolonged PT or APTT found
  2. 2Mix patient plasma 1:1 with normal plasma
  3. 3Repeat the test
  4. 4Corrects

    Factor deficiency likely

  5. 5Does not correct

    Inhibitor present, e.g. lupus anticoagulant

Study guide

In this practical you prepare and stain bone marrow smears (Leishman, May-Grunwald-Giemsa, Perl's), section and stain trephine biopsies (H&E, reticulin, PAS), identify immature cells, study red cell abnormalities, perform coagulation mixing studies, examine urine, CSF and seminal fluid (including sperm count) and demonstrate blood cell counters and coagulometers. Practise recognising the maturation sequence of each cell line.

Why Applied Haematology-I (Practical) Matters

These advanced haematology skills are in demand in hospital haematology and oncology laboratories.

How to Prepare for Applied Haematology-I (Practical)

  • Study atlas images alongside your slides.
  • Practise differential counting on marrow smears.
  • Handle body fluids with full safety precautions.

Applied Haematology-I (Practical): Frequently Asked Questions

What is done in the applied haematology-I practical?

Marrow smear and biopsy staining, cell identification, coagulation mixing studies and body fluid examination.

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