Applied Haematology-II (Practical) Notes
Subject Overview
The practical companion to Applied Haematology-II, testing for G6PD deficiency, HbF and abnormal haemoglobin, red cell osmotic fragility, cytochemical staining and platelet function. A 1-credit skill enhancement laboratory paper.
Subject at a glance
1
Units
9
Topics
1
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Haemoglobin and platelet function testing
- G6PD deficiency detection
- Hb-F estimation
- plasma and urine haemoglobin estimation
- Hb-S sickling and solubility tests
- osmotic red cell fragility test
- cytochemical staining (PAS, SBB, MPO, LAP, Perl's reaction)
- platelet function tests — whole blood clot retraction
- prothrombin consumption index
- platelet adhesion/aggregation and PF3 availability
MPO (myeloperoxidase)
Myeloid cells
AML from ALL
SBB (Sudan black B)
Myeloid cells
AML from ALL
PAS
Lymphoblasts (block positivity)
ALL; erythroleukaemia
LAP score
Mature neutrophils
Low in CML, high in leukaemoid reaction
Perl's stain
Iron stores
Iron deficiency, sideroblastic anaemia
Study guide
In this practical you detect G6PD deficiency, estimate HbF, plasma and urine haemoglobin, perform sickling and solubility tests, osmotic fragility, cytochemical stains (PAS, SBB, MPO, LAP, Perl's) and platelet function tests (clot retraction, prothrombin consumption, adhesion, aggregation and PF3 availability). Use positive and negative controls for each test.
Why Applied Haematology-II (Practical) Matters
These special tests support diagnosis of inherited haemoglobin and enzyme disorders and leukaemias.
How to Prepare for Applied Haematology-II (Practical)
- Learn the principle and interpretation of each test.
- Practise cytochemical staining on known slides.
- Always run controls with special tests.
Applied Haematology-II (Practical): Frequently Asked Questions
What is a sickling test?
It is a test that detects sickle haemoglobin by exposing red cells to low oxygen conditions and looking for sickle-shaped cells.