Basic Haematological Techniques-II (Practical) Notes
Subject Overview
The practical companion to Basic Haematological Techniques-II, measuring abnormal haemoglobin pigments, platelet count by phase-contrast microscopy, coagulation screening (PT, PTI, INR, APTT) and preparing the reagents used in coagulation testing. A 2-credit core practical paper.
Subject at a glance
1
Units
10
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Haemoglobin and coagulation testing
- Measurement of methaemoglobin
- carboxyhaemoglobin and sulphahaemoglobin levels
- platelet count by phase-contrast microscopy
- PT
- PTI
- INR and APTT determination
- laboratory preparation of brain thromboplastin
- cephalin
- thrombin
- M/40 CaCl2 and kaolin solution
PT ratio
Patient PT / control (mean normal) PT
INR
(Patient PT / mean normal PT)^ISI
ISI comes with the thromboplastin reagent
Prothrombin index
(Control PT / patient PT) × 100
Target INR on warfarin
Usually 2.0–3.0
Study guide
In this practical you measure methaemoglobin, carboxyhaemoglobin and sulphaemoglobin, count platelets by phase-contrast microscopy, perform PT, PTI, INR and APTT and prepare reagents such as brain thromboplastin, cephalin, thrombin, calcium chloride and kaolin. Understand how INR standardises PT results across laboratories.
Why Basic Haematological Techniques-II (Practical) Matters
Coagulation testing and INR monitoring are critical for patients on anticoagulants, making these skills in high demand in hospitals.
How to Prepare for Basic Haematological Techniques-II (Practical)
- Time reactions accurately with a stopwatch.
- Record PT, PTI and INR with the normal control.
- Prepare reagents exactly as per protocol.
Basic Haematological Techniques-II (Practical): Frequently Asked Questions
What is INR?
The International Normalised Ratio standardises prothrombin time results so that they can be compared between laboratories, and is used to monitor warfarin therapy.