Basics of Biochemistry (Practical) Notes
Subject Overview
The practical companion to Basics of Biochemistry, covering glassware cleaning, distilled water preparation, pH meter operation, and the preparation of standard reagent solutions (NaOH, HCl, H2SO4, sodium carbonate) used throughout the biochemistry laboratory. A 2-credit core practical paper.
Subject at a glance
1
Units
6
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Laboratory technique and reagent preparation
- Cleaning of laboratory glassware
- preparation of distilled water
- principle/working/maintenance of the pH meter
- preparation of 0.1N NaOH
- 0.2N HCl
- 0.1 molar H2SO4 and 0.2 molar sodium carbonate solutions
0.1 N NaOH
4 g NaOH per litre
Molecular weight 40
0.2 N HCl
About 16.6 mL of concentrated HCl per litre (approx.)
0.1 M H2SO4
About 5.4 mL of concentrated acid per litre (approx.)
Add acid to water, never water to acid
0.2 M Na2CO3
21.2 g anhydrous per litre
Molecular weight 106
Study guide
In this practical you clean glassware, prepare distilled water, learn the principle, working and maintenance of the pH meter and prepare standard solutions such as 0.1N NaOH, 0.2N HCl, 0.1 M sulphuric acid and 0.2 M sodium carbonate. Weigh and measure carefully; small errors multiply in solution preparation.
Why Basics of Biochemistry (Practical) Matters
Correct preparation of reagents and standard solutions is the first step of every accurate laboratory test.
How to Prepare for Basics of Biochemistry (Practical)
- Show your calculation for each solution in the record.
- Calibrate the pH meter before each use.
- Add acid to water, never the reverse.
Basics of Biochemistry (Practical): Frequently Asked Questions
What is a standard solution?
A standard solution is a solution of accurately known concentration.