Biochemical Metabolism (Practical) Notes
Subject Overview
The practical companion to Biochemical Metabolism: qualitative tests for carbohydrates and reducing sugars (Molisch, Fehling, Benedict's, Iodine), and quantitative estimation of glucose, urea, creatinine, albumin and cholesterol in serum, plasma and urine. A 2-credit core practical paper.
Subject at a glance
1
Units
8
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Qualitative and quantitative biochemical estimation
- Molisch test for carbohydrates
- Fehling and Benedict's tests for reducing sugar
- Iodine test for starch
- estimation of glucose by Folin & Wu method
- estimation of urea in serum/plasma/urine
- estimation of creatinine
- estimation of serum albumin
- estimation of cholesterol in serum or plasma
Molisch test
Purple ring at the junction
All carbohydrates
Benedict's test
Green to brick-red precipitate
Reducing sugars
Fehling's test
Red precipitate
Reducing sugars
Iodine test
Blue-black colour
Starch
Study guide
In this practical you carry out qualitative tests (Molisch, Fehling, Benedict, iodine) and quantitative estimates: glucose by the Folin and Wu method, urea, creatinine, serum albumin and cholesterol. Prepare standards and blanks carefully for each estimation.
Why Biochemical Metabolism (Practical) Matters
Glucose, urea, creatinine, albumin and cholesterol are among the most frequently ordered clinical tests, so mastering them is directly job-relevant.
How to Prepare for Biochemical Metabolism (Practical)
- Pipette accurately and record readings carefully.
- Learn the normal reference ranges of each test.
- Plot a calibration curve neatly where required.
Biochemical Metabolism (Practical): Frequently Asked Questions
What tests are done in the biochemical metabolism practical?
Qualitative carbohydrate tests and quantitative estimation of glucose, urea, creatinine, albumin and cholesterol.