NotesB.Sc MLSClinical Biochemistry-1 (Practical)
BMLS406-18

Clinical Biochemistry-1 (Practical) Notes

Program:B.Sc MLS
Semester:Semester 4
Credits:2
Units:1 Units

Subject Overview

The practical companion to Clinical Biochemistry-1, estimating glucose, protein, urea, uric acid, bilirubin, cholesterol fractions (total, HDL, LDL), triglycerides, creatinine, calcium and electrolytes in blood and urine samples. A 2-credit core practical paper.

Subject at a glance

1

Units

11

Topics

2

Credits

1

Infographics

Unit-wise Syllabus

1 units — click WhatsApp below to get the full notes for each

1

Unit 1: Biochemical panel estimation

  • Estimation of glucose and protein in urine and blood
  • urea
  • uric acid
  • serum bilirubin
  • total cholesterol
  • HDL cholesterol
  • LDL cholesterol
  • triglycerides
  • creatinine and serum calcium
  • measurement of sodium
  • potassium and chloride electrolytes
Key formulasLipid profile calculations
  • Friedewald LDL

    LDL = total cholesterol − HDL − (triglycerides / 5)

    Valid when triglycerides < 400 mg/dL

  • Non-HDL cholesterol

    Total cholesterol − HDL

  • Desirable total cholesterol

    < 200 mg/dL

  • Desirable HDL

    > 40 mg/dL (men), > 50 mg/dL (women)

Study guide

In this practical you estimate glucose and protein in urine and blood, urea, uric acid, serum bilirubin, total, HDL and LDL cholesterol, triglycerides, creatinine and serum calcium and measure electrolytes. Prepare standards and blanks carefully, and always check results against reference ranges.

Why Clinical Biochemistry-1 (Practical) Matters

These are the routine investigations of every hospital biochemistry laboratory, so proficiency here is directly employable.

How to Prepare for Clinical Biochemistry-1 (Practical)

  • Learn the reference range and significance of every test.
  • Pipette accurately and record absorbance readings carefully.
  • Use controls to verify each run.

Clinical Biochemistry-1 (Practical): Frequently Asked Questions

What tests are done in the Clinical Biochemistry-1 practical?

Glucose, protein, urea, uric acid, bilirubin, lipid profile, creatinine, calcium and electrolytes.

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