Clinical Biochemistry-1 (Practical) Notes
Subject Overview
The practical companion to Clinical Biochemistry-1, estimating glucose, protein, urea, uric acid, bilirubin, cholesterol fractions (total, HDL, LDL), triglycerides, creatinine, calcium and electrolytes in blood and urine samples. A 2-credit core practical paper.
Subject at a glance
1
Units
11
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Biochemical panel estimation
- Estimation of glucose and protein in urine and blood
- urea
- uric acid
- serum bilirubin
- total cholesterol
- HDL cholesterol
- LDL cholesterol
- triglycerides
- creatinine and serum calcium
- measurement of sodium
- potassium and chloride electrolytes
Friedewald LDL
LDL = total cholesterol − HDL − (triglycerides / 5)
Valid when triglycerides < 400 mg/dL
Non-HDL cholesterol
Total cholesterol − HDL
Desirable total cholesterol
< 200 mg/dL
Desirable HDL
> 40 mg/dL (men), > 50 mg/dL (women)
Study guide
In this practical you estimate glucose and protein in urine and blood, urea, uric acid, serum bilirubin, total, HDL and LDL cholesterol, triglycerides, creatinine and serum calcium and measure electrolytes. Prepare standards and blanks carefully, and always check results against reference ranges.
Why Clinical Biochemistry-1 (Practical) Matters
These are the routine investigations of every hospital biochemistry laboratory, so proficiency here is directly employable.
How to Prepare for Clinical Biochemistry-1 (Practical)
- Learn the reference range and significance of every test.
- Pipette accurately and record absorbance readings carefully.
- Use controls to verify each run.
Clinical Biochemistry-1 (Practical): Frequently Asked Questions
What tests are done in the Clinical Biochemistry-1 practical?
Glucose, protein, urea, uric acid, bilirubin, lipid profile, creatinine, calcium and electrolytes.
