Histotechnology-I (Practical)
Subject Overview
The practical companion to Histotechnology-I, taking a specimen from reception through fixative preparation, decalcification, embedding, section cutting, mounting and H&E/PTAH staining — the complete manual histopathology workflow. A 2-credit elective laboratory paper.
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: The full tissue-processing workflow
Dissection instruments, antiseptics/disinfectants/insecticides in tissue processing, specimen reception and labelling, preparation of fixatives (Helly's, Zenker's, Bouin's, Corney's, neutral formalin, formal saline, formal acetic acid, Perenyi's fluid), embedding and block casting, bone decalcification, preparing 70% alcohol, manual and automated tissue processing, microtome parts and types, knife sharpening (honing and stropping), section cutting and mounting, tissue floatation bath and section drying, Haematoxylin and Eosin staining, PTAH staining, mounting stained smears