Immunology and Mycology (Practical) Notes
Subject Overview
The practical companion to Immunology and Mycology, performing the serology and mycology bench work — serum separation, the Widal/
Subject at a glance
1
Units
14
Topics
1
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Serology and mycology bench work
- Blood collection by venipuncture and serum separation
- Widal
- VDRL (with antigen preparation)
- ASO
- CRP and RF serological tests
- immunofluorescence
- immunodiffusion (Ouchterlony)
- CCIEP
- ELISA
- SDS-PAGE and western blotting demonstration
- culture media preparation for mycology
- fungal staining and identification (yeast and mould cultures)
- demonstration of fungal dimorphism
- processing clinical samples (skin, nail, hair, body fluids) for fungal diagnosis
- 1
Collect scrapings or clippings
- 2
KOH mount
10–20% KOH dissolves keratin
- 3
Look for hyphae or yeast cells
- 4
Culture on Sabouraud dextrose agar
- 5
Incubate at 25–30 °C
Up to several weeks
- 6
Identify
Colony and LPCB mount morphology
Study guide
In this practical you collect blood by venipuncture and separate serum, perform Widal, VDRL, ASO, CRP and RF tests, demonstrate immunofluorescence, Ouchterlony immunodiffusion, CCIEP, ELISA, SDS-PAGE and western blotting, prepare mycology media, stain and identify yeasts and moulds, demonstrate fungal dimorphism and process skin, nail, hair and fluid samples. Follow strict safety rules when handling blood and serum.
Why Immunology and Mycology (Practical) Matters
Serology and mycology are routine services in diagnostic laboratories and a source of specialised employment.
How to Prepare for Immunology and Mycology (Practical)
- Practise correct venipuncture and serum separation technique.
- Interpret agglutination results carefully and use controls.
- Learn to recognise common fungi under the microscope.
Immunology and Mycology (Practical): Frequently Asked Questions
What is done in the immunology and mycology practical?
Serological tests, demonstrations of immunological techniques and fungal culture, staining and identification.