Systemic Bacteriology (Practical) Notes
Subject Overview
The practical companion to Systemic Bacteriology: bacterial culture technique and media preparation, the Gram, Albert, Z-N and capsule staining methods, positive/
Subject at a glance
1
Units
20
Topics
2
Credits
1
Infographics
Unit-wise Syllabus
1 units — click WhatsApp below to get the full notes for each
Unit 1: Culture, staining and identification technique
- Bacterial culture technique and media isolation
- Gram
- Albert
- Z-N and capsule staining
- biochemical tests with positive and negative controls — catalase
- coagulase
- indole
- MR
- VP
- urease
- citrate
- oxidase
- TSI
- nitrate reduction
- carbohydrate fermentation
- Hugh and Leifson
- bile solubility
- H2S production
- collection and transport of clinical samples
- characterization of commonly isolated pathogens (Staphylococcus, E. coli, Klebsiella, Proteus, M. tuberculosis, Pseudomonas)
- 1
Collect the sample
Correct container, before antibiotics
- 2
Transport promptly
Transport media if needed
- 3
Direct smear
Gram or Z-N stain
- 4
Culture
Blood agar, MacConkey, etc.
- 5
Colony morphology
- 6
Biochemical tests
With positive and negative controls
- 7
Report the identification
Study guide
In this practical you perform culture and media isolation, Gram, Albert, Ziehl-Neelsen and capsule stains, biochemical tests with positive and negative controls, sample collection and transport and characterise common pathogens such as Staphylococcus, E. coli, Klebsiella, Proteus, Mycobacterium tuberculosis and Pseudomonas. Always run controls; a test result without controls cannot be trusted.
Why Systemic Bacteriology (Practical) Matters
These practical skills are the core daily routine of a clinical microbiology technologist.
How to Prepare for Systemic Bacteriology (Practical)
- Record each biochemical result in a table with controls.
- Practise staining on known and unknown smears.
- Follow biosafety rules strictly with pathogens.
Systemic Bacteriology (Practical): Frequently Asked Questions
What tests are done in the systemic bacteriology practical?
Culture, Gram and special stains and a battery of biochemical tests to identify common pathogens.